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Oral delivery of cyclosporin A (CsA) via lipid nanoparticles (LNPs) has the potential to improve bioavailability and overcome poor oral absorption of the drug. In this study, LNPs of phospholipids were prepared by electrospray method and their characterization has been done by particle size, zeta potential, and polydispersity index. We have carried out in vitro release of the drug in simulated gastrointestinal juices and simulated intestinal fluids. The drug release was significantly retarded at pH 1.2 and 7.4 compared to that at pH 6.8, indicating that LNPs protected the drug from degradation by gastric acid. LNPs displayed in vitro release half-life of 7 and 60 min in simulated gastrointestinal juice at pH 1.2 and 7.4, respectively. These values decreased drastically to 15 min and 1 min in simulated intestinal fluid at pH 6.8 and 7.4, respectively. Further, cytotoxicity was evaluated by (3-(4,5-dimethylthiazol-2-yl)- 0b46394aab